In the First Stage of Cell Reproduction What Disappears

In the First Stage of Cell Reproduction What Disappears.

6.2: The Cell Cycle

  • Folio ID
  • The cell bike is an ordered series of events involving prison cell growth and prison cell division that produces two new daughter cells. Cells on the path to cell partition go on through a series of precisely timed and carefully regulated stages of growth, Dna replication, and partitioning that produce two genetically identical cells. The jail cell cycle has 2 major phases: interphase and the mitotic phase (Effigy \(\PageIndex{i}\)). During interphase, the cell grows and Deoxyribonucleic acid is replicated. During the mitotic stage, the replicated DNA and cytoplasmic contents are separated and the cell divides.

    This illustration shows the cell cycle, which consists of interphase and the mitotic phase. Interphase is subdivided into G1, S, and G2 phases. Cell growth occurs during G1 and G2, and DNA synthesis occurs during S. The mitotic phase consists of mitosis, in which the nuclear chromatin is divided, and cytokinesis, in which the cytoplasm is divided resulting in two daughter cells.
    Figure \(\PageIndex{ane}\):
    A cell moves through a series of phases in an orderly manner. During interphase, G1
    involves cell growth and protein synthesis, the S stage involves DNA replication and the replication of the centrosome, and One thousand2
    involves further growth and protein synthesis. The mitotic phase follows interphase. Mitosis is nuclear partition during which duplicated chromosomes are segregated and distributed into daughter nuclei. Usually the jail cell will split up after mitosis in a process called cytokinesis in which the cytoplasm is divided and two daughter cells are formed.


    During interphase, the prison cell undergoes normal processes while also preparing for cell sectionalization. For a cell to move from interphase to the mitotic phase, many internal and external weather condition must be met. The three stages of interphase are called Gone, South, and G2.


    The beginning phase of interphase is chosen the G1
    phase, or first gap, because trivial change is visible. Even so, during the Gi
    phase, the prison cell is quite active at the biochemical level. The jail cell is accumulating the building blocks of chromosomal Deoxyribonucleic acid and the associated proteins, as well as accumulating plenty free energy reserves to complete the task of replicating each chromosome in the nucleus.

    S Stage

    Throughout interphase, nuclear DNA remains in a semi-condensed chromatin configuration. In the S phase (synthesis phase), DNA replication results in the formation of two identical copies of each chromosome—sister chromatids—that are firmly attached at the centromere region. At this stage, each chromosome is made of two sister chromatids and is a duplicated chromosome. The centrosome is duplicated during the S phase. The 2 centrosomes will give rise to the mitotic spindle, the apparatus that orchestrates the motion of chromosomes during mitosis. The centrosome consists of a pair of rod-like centrioles at right angles to each other. Centrioles help organize cell division. Centrioles are not nowadays in the centrosomes of many eukaryotic species, such as plants and most fungi.


    In the One thousand2
    phase, or 2nd gap, the cell replenishes its energy stores and synthesizes the proteins necessary for chromosome manipulation. Some cell organelles are duplicated, and the cytoskeleton is dismantled to provide resources for the mitotic spindle. There may be additional cell growth during Gtwo. The final preparations for the mitotic phase must exist completed before the cell is able to enter the first stage of mitosis.

    The Mitotic Stage

    To brand two daughter cells, the contents of the nucleus and the cytoplasm must be divided. The mitotic stage is a multistep process during which the duplicated chromosomes are aligned, separated, and moved to contrary poles of the cell, and then the cell is divided into ii new identical daughter cells. The start portion of the mitotic stage, mitosis, is composed of five stages, which reach nuclear sectionalization. The second portion of the mitotic stage, called cytokinesis, is the physical separation of the cytoplasmic components into ii daughter cells.


    Mitosis is divided into a series of phases—prophase, prometaphase, metaphase, anaphase, and telophase—that result in the partition of the cell nucleus (Figure \(\PageIndex{ii}\)).

    ART Connexion

    This diagram shows the five phases of mitosis, and cytokinesis. During prophase, the chromosomes condense and become visible, spindle fibers emerge from the centrosomes, the centrosomes move toward opposite poles, and the nuclear envelope breaks down. During prometaphase, the chromosomes continue to condense and kinetochores appear at the centromeres. Mitotic spindle microtubules attach to the kinetochores. During metaphase, the centrosomes are at opposite poles of the cell. Chromosomes line up at the metaphase plate and each sister chromatid is attached to spindle fibers originating from the opposite poles. During anaphase, the centromeres split in two. The sister chromatids, which are now called chromosomes, move toward opposite poles of the cell. Certain spindle fibers lengthen, elongating the cell. During telophase, the chromosomes arrive at the opposite poles and begin to decondense. The nuclear envelope re-forms. During cytokinesis in animals, a cleavage furrow separates the two daughter cells. In plants, a cell plate—the precursor to a new cell wall—separates the two daughter cells.
    Figure \(\PageIndex{two}\):
    Animal cell mitosis is divided into five stages—prophase, prometaphase, metaphase, anaphase, and telophase—visualized hither past lite microscopy with fluorescence. Mitosis is usually accompanied by cytokinesis, shown hither past a manual electron microscope. (credit “diagrams”: modification of piece of work past Mariana Ruiz Villareal; credit “mitosis micrographs”: modification of work by Roy van Heesbeen; credit “cytokinesis micrograph”: modification of piece of work past the Wadsworth Heart, NY State Section of Wellness; donated to the Wikimedia foundation; calibration-bar data from Matt Russell)

    Which of the following is the correct lodge of events in mitosis?

    1. Sister chromatids line up at the metaphase plate. The kinetochore becomes attached to the mitotic spindle. The nucleus re-forms and the cell divides. The sister chromatids dissever.
    2. The kinetochore becomes attached to the mitotic spindle. The sis chromatids separate. Sister chromatids line up at the metaphase plate. The nucleus re-forms and the cell divides.
    3. The kinetochore becomes fastened to metaphase plate. Sister chromatids line upwardly at the metaphase plate. The kinetochore breaks downward and the sister chromatids separate. The nucleus re-forms and the cell divides.
    4. The kinetochore becomes attached to the mitotic spindle. Sister chromatids line up at the metaphase plate. The kinetochore breaks apart and the sister chromatids separate. The nucleus re-forms and the prison cell divides.

    During prophase, the “first stage,” several events must occur to provide access to the chromosomes in the nucleus. The nuclear envelope starts to break into small vesicles, and the Golgi appliance and endoplasmic reticulum fragment and disperse to the periphery of the jail cell. The nucleolus disappears. The centrosomes begin to movement to reverse poles of the cell. The microtubules that course the basis of the mitotic spindle extend between the centrosomes, pushing them farther autonomously as the microtubule fibers lengthen. The sister chromatids begin to gyre more tightly and get visible nether a light microscope.

    During prometaphase, many processes that were begun in prophase go along to advance and culminate in the formation of a connection between the chromosomes and cytoskeleton. The remnants of the nuclear envelope disappear. The mitotic spindle continues to develop as more microtubules assemble and stretch beyond the length of the quondam nuclear area. Chromosomes become more than condensed and visually detached. Each sister chromatid attaches to spindle microtubules at the centromere via a protein complex called the kinetochore.

    During metaphase, all of the chromosomes are aligned in a airplane called the metaphase plate, or the equatorial plane, midway between the two poles of the cell. The sister chromatids are still tightly attached to each other. At this time, the chromosomes are maximally condensed.

    During anaphase, the sister chromatids at the equatorial aeroplane are split apart at the centromere. Each chromatid, now called a chromosome, is pulled rapidly toward the centrosome to which its microtubule was attached. The cell becomes visibly elongated as the non-kinetochore microtubules slide against each other at the metaphase plate where they overlap.

    During telophase, all of the events that ready the duplicated chromosomes for mitosis during the first three phases are reversed. The chromosomes reach the contrary poles and begin to decondense (unravel). The mitotic spindles are broken down into monomers that volition be used to assemble cytoskeleton components for each daughter prison cell. Nuclear envelopes grade around chromosomes.

    CONCEPT IN Activity

    This page of movies illustrates unlike aspects of mitosis. Picket the film entitled “DIC microscopy of cell division in a newt lung cell” and identify the phases of mitosis.


    Cytokinesis is the second part of the mitotic stage during which cell division is completed by the physical separation of the cytoplasmic components into two girl cells. Although the stages of mitosis are like for most eukaryotes, the process of cytokinesis is quite unlike for eukaryotes that have prison cell walls, such equally plant cells.

    In cells such equally animal cells that lack cell walls, cytokinesis begins following the onset of anaphase. A contractile ring composed of actin filaments forms just inside the plasma membrane at the former metaphase plate. The actin filaments pull the equator of the jail cell inward, forming a fissure. This crevice, or “scissure,” is called the cleavage furrow. The furrow deepens as the actin ring contracts, and eventually the membrane and prison cell are cleaved in 2 (Figure \(\PageIndex{iii}\)).

    In institute cells, a cleavage furrow is not possible considering of the rigid jail cell walls surrounding the plasma membrane. A new cell wall must grade between the daughter cells. During interphase, the Golgi apparatus accumulates enzymes, structural proteins, and glucose molecules prior to breaking upward into vesicles and dispersing throughout the dividing cell. During telophase, these Golgi vesicles move on microtubules to collect at the metaphase plate. At that place, the vesicles fuse from the eye toward the cell walls; this structure is chosen a cell plate. Every bit more vesicles fuse, the cell plate enlarges until it merges with the cell wall at the periphery of the cell. Enzymes use the glucose that has accumulated between the membrane layers to build a new prison cell wall of cellulose. The Golgi membranes become the plasma membrane on either side of the new prison cell wall (Effigy \(\PageIndex{3}\)).

    This illustration shows cytokinesis in a typical animal cell and a typical plant cell. In an animal cell, a contractile ring of actin filaments forms a cleavage furrow that divides the cell in two. In a plant cell, Golgi vesicles coalesce at the metaphase plate. A cell plate grows from the center outward, and the vesicles form a plasma membrane that divides the cytoplasm.
    Figure \(\PageIndex{iii}\):
    In part (a), a cleavage furrow forms at the former metaphase plate in the fauna cell. The plasma membrane is drawn in by a ring of actin fibers contracting simply inside the membrane. The cleavage furrow deepens until the cells are pinched in two. In role (b), Golgi vesicles coalesce at the onetime metaphase plate in a plant prison cell. The vesicles fuse and class the prison cell plate. The prison cell plate grows from the center toward the jail cell walls. New cell walls are made from the vesicle contents.


    Not all cells attach to the classic cell-cycle pattern in which a newly formed daughter prison cell immediately enters interphase, closely followed past the mitotic stage. Cells in the K0
    phase are not actively preparing to dissever. The jail cell is in a quiescent (inactive) stage, having exited the cell cycle. Some cells enter Yard0
    temporarily until an external signal triggers the onset of Yard1. Other cells that never or rarely split, such as mature cardiac muscle and nerve cells, remain in M0
    permanently (Effigy \(\PageIndex{iv}\)).

    The cell cycle is shown in a circular graphic, with four stages. The S stage accounts for about 40 percent of the cycle. The G2 stage accounts for about 19 percent. Mitosis accounts for 2 percent, and G1 accounts for 39 percent. An arrow is shown exiting the G1 stage that points to the G0 stage outside the circle, in which cells are not actively dividing. Another arrow points from the G0 stage back into the G1 stage, where cells may re-enter the cycle.
    Figure \(\PageIndex{four}\): Cells that are non actively preparing to dissever enter an alternate phase called Yard0. In some cases, this is a temporary condition until triggered to enter G1. In other cases, the cell volition remain in G0

    Control of the Cell Cycle

    The length of the cell cycle is highly variable even within the cells of an individual organism. In humans, the frequency of jail cell turnover ranges from a few hours in early embryonic evolution to an average of two to five days for epithelial cells, or to an entire human being lifetime spent in K0
    by specialized cells such as cortical neurons or cardiac muscle cells. There is also variation in the time that a cell spends in each phase of the cell bike. When fast-dividing mammalian cells are grown in culture (exterior the torso under optimal growing conditions), the length of the bike is approximately 24 hours. In speedily dividing man cells with a 24-hour cell wheel, the G1
    phase lasts approximately 11 hours. The timing of events in the cell bicycle is controlled by mechanisms that are both internal and external to the cell.

    Regulation at Internal Checkpoints

    It is essential that daughter cells exist exact duplicates of the parent prison cell. Mistakes in the duplication or distribution of the chromosomes atomic number 82 to mutations that may be passed forward to every new jail cell produced from the aberrant jail cell. To preclude a compromised jail cell from standing to divide, there are internal control mechanisms that operate at three principal prison cell bicycle checkpoints at which the cell cycle can be stopped until conditions are favorable. These checkpoints occur near the finish of Gi, at the One thousand2–M transition, and during metaphase (Figure \(\PageIndex{5}\)).

    This illustration shows the three major check points of the cell cycle, which occur in G1, G2, and mitosis.
    Figure \(\PageIndex{v}\):
    The cell bicycle is controlled at three checkpoints. Integrity of the DNA is assessed at the G1
    checkpoint. Proper chromosome duplication is assessed at the Thouii
    checkpoint. Attachment of each kinetochore to a spindle fiber is assessed at the M checkpoint.

    The Gane

    The G1
    checkpoint determines whether all conditions are favorable for cell division to proceed. The K1
    checkpoint, too called the restriction point, is the point at which the cell irreversibly commits to the cell-division process. In add-on to adequate reserves and jail cell size, there is a check for damage to the genomic DNA at the G1
    checkpoint. A cell that does not meet all the requirements will not exist released into the S phase.

    The G2

    The Grand2
    checkpoint bars the entry to the mitotic stage if certain conditions are non met. As in the G1
    checkpoint, cell size and poly peptide reserves are assessed. However, the most important role of the Yard2
    checkpoint is to ensure that all of the chromosomes have been replicated and that the replicated DNA is not damaged.

    The Grand Checkpoint

    The M checkpoint occurs near the cease of the metaphase stage of mitosis. The Thou checkpoint is too known as the spindle checkpoint because it determines if all the sis chromatids are correctly attached to the spindle microtubules. Because the separation of the sister chromatids during anaphase is an irreversible step, the cycle will not proceed until the kinetochores of each pair of sister chromatids are firmly anchored to spindle fibers arising from opposite poles of the cell.


    Watch what occurs at the G1, Grandii, and M checkpoints by visiting this animation of the cell wheel.


    The cell bike is an orderly sequence of events. Cells on the path to jail cell division go along through a series of precisely timed and carefully regulated stages. In eukaryotes, the cell cycle consists of a long preparatory period, called interphase. Interphase is divided into Thou1, South, and M2
    phases. Mitosis consists of five stages: prophase, prometaphase, metaphase, anaphase, and telophase. Mitosis is ordinarily accompanied by cytokinesis, during which the cytoplasmic components of the daughter cells are separated either past an actin ring (brute cells) or past jail cell plate formation (plant cells).

    Each step of the prison cell cycle is monitored past internal controls called checkpoints. There are three major checkpoints in the cell bike: 1 near the terminate of Gane, a second at the G2–Grand transition, and the third during metaphase.

    Art Connections

    Effigy \(\PageIndex{2}\): Which of the following is the right society of events in mitosis?

    1. Sis chromatids line up at the metaphase plate. The kinetochore becomes attached to the mitotic spindle. The nucleus re-forms and the cell divides. The sister chromatids separate.
    2. The kinetochore becomes attached to the mitotic spindle. The sister chromatids split up. Sister chromatids line upwardly at the metaphase plate. The nucleus re-forms and the prison cell divides.
    3. The kinetochore becomes attached to metaphase plate. Sister chromatids line upward at the metaphase plate. The kinetochore breaks down and the sis chromatids separate. The nucleus re-forms and the prison cell divides.
    4. The kinetochore becomes fastened to the mitotic spindle. Sister chromatids line upwardly at the metaphase plate. The kinetochore breaks apart and the sis chromatids separate. The nucleus re-forms and the cell divides.

    D. The kinetochore becomes fastened to the mitotic spindle. Sis chromatids line up at the metaphase plate. The kinetochore breaks apart and the sis chromatids split up. The nucleus reforms and the cell divides.


    the stage of mitosis during which sister chromatids are separated from each other
    cell bike
    the ordered sequence of events that a cell passes through between i jail cell division and the next
    jail cell wheel checkpoints
    mechanisms that monitor the preparedness of a eukaryotic cell to advance through the various cell cycle stages
    cell plate
    a structure formed during establish-prison cell cytokinesis by Golgi vesicles fusing at the metaphase plate; will ultimately lead to germination of a jail cell wall to separate the two girl cells
    a paired rod-like construction synthetic of microtubules at the center of each brute jail cell centrosome
    cleavage furrow
    a constriction formed by the actin ring during animal-cell cytokinesis that leads to cytoplasmic segmentation
    the division of the cytoplasm following mitosis to form two daughter cells
    a prison cell-cycle phase distinct from the G1
    phase of interphase; a cell in G0
    is non preparing to split up
    (also, kickoff gap) a prison cell-cycle phase; outset phase of interphase centered on cell growth during mitosis
    (also, second gap) a cell-bicycle phase; third phase of interphase where the cell undergoes the concluding preparations for mitosis
    the menstruation of the cell wheel leading up to mitosis; includes One thousand1, S, and One thousandtwo
    phases; the interim between two consecutive cell divisions
    a protein structure in the centromere of each sis chromatid that attracts and binds spindle microtubules during prometaphase
    metaphase plate
    the equatorial plane midway between two poles of a cell where the chromosomes marshal during metaphase
    the stage of mitosis during which chromosomes are lined up at the metaphase plate
    the menstruum of the cell cycle at which the duplicated chromosomes are separated into identical nuclei; includes prophase, prometaphase, metaphase, anaphase, and telophase
    mitotic stage
    the period of the cell cycle when duplicated chromosomes are distributed into 2 nuclei and the cytoplasmic contents are divided; includes mitosis and cytokinesis
    mitotic spindle
    the microtubule apparatus that orchestrates the movement of chromosomes during mitosis
    the phase of mitosis during which mitotic spindle fibers attach to kinetochores
    the stage of mitosis during which chromosomes condense and the mitotic spindle begins to course
    describes a jail cell that is performing normal cell functions and has non initiated preparations for jail cell segmentation
    S phase
    the 2d, or synthesis phase, of interphase during which Deoxyribonucleic acid replication occurs
    the stage of mitosis during which chromosomes arrive at reverse poles, decondense, and are surrounded by new nuclear envelopes

    Contributors and Attributions

    • Samantha Fowler (Clayton State Academy), Rebecca Roush (Sandhills Customs College), James Wise (Hampton University). Original content by OpenStax (CC BY 4.0; Access for free at…iv-e119a8aafbdd).

    In the First Stage of Cell Reproduction What Disappears