The Ph Indicator Bromothymol Blue is Blue

The Ph Indicator Bromothymol Blue is Blue.

pH indicator

Bromothymol blue
Bromothymol-blue-2D-skeletal.png
Bromothymol-blue-3D-balls.png
Names
Preferred IUPAC name

3,3-Bis[iii-bromo-4-hydroxy-two-methyl-five-(propan-ii-yl)phenyl]-2,1λ6-benzoxathiole-one,1(3H)-dione

Identifiers

CAS Number

  • 76-59-5
    check
    Y

3D model (JSmol)

  • Interactive image
ChEBI
  • CHEBI:86155
    ☒
    N
ChEMBL
  • ChEMBL3392578
ChemSpider
  • 6208
    check
    Y
ECHA InfoCard 100.000.884
Edit this at Wikidata
EC Number
  • 200-971-2

PubChem
CID

  • 6450
UNII
  • VGU4LM0H96
    check
    Y

CompTox Dashboard
(EPA)

  • DTXSID3058799
    Edit this at Wikidata

InChI

  • InChI=1S/C27H28Br2O5S/c1-13(2)17-11-20(xv(5)23(28)25(17)30)27(19-9-seven-eight-ten-22(xix)35(32,33)34-27)21-12-18(14(iii)four)26(31)24(29)16(21)half dozen/h7-14,30-31H,ane-6H3check
    Y

    Key: NUHCTOLBWMJMLX-UHFFFAOYSA-Ncheck
    Y

  • InChI=1/C27H28Br2O5S/c1-13(2)17-11-20(fifteen(5)23(28)25(17)xxx)27(xix-9-7-8-ten-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-fourteen,30-31H,1-6H3

    Central: NUHCTOLBWMJMLX-UHFFFAOYAD

SMILES

  • Brc1c(O)c(cc(c1C)C3(Bone(=O)(=O)c2ccccc23)c4cc(c(O)c(Br)c4C)C(C)C)C(C)C

Properties

Chemic formula

C
27
H
28
Br
2
O
5
South
Molar mass
624.38
 g·mol−1
Density 1.25 yard/cm3
Melting point 202 °C (396 °F; 475 M)

Solubility in water

Sparingly soluble in h2o[1]
Acerbity (pK
a)
7.0
Hazards
GHS
labelling:

Pictograms

GHS07: Exclamation mark

Signal word

Warning

Run a risk statements

H302,
H315,
H319

Precautionary statements

P264,
P270,
P280,
P301+P312,
P302+P352,
P305+P351+P338,
P321,
P330,
P332+P313,
P337+P313,
P362,
P501
NFPA 704
(fire diamond)

2

one

0

Safety data sheet (SDS) http://www.sciencelab.com/msds.php?msdsId=9927468

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

☒
Northverify (what is
check
Y
☒
N
 ?)

Infobox references

Chemical chemical compound

Bromothymol blueish
(besides known as
bromothymol sulfone phthalein
and
BTB) is a pH indicator. Information technology is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). A common use is for measuring the presence of carbonic acrid in a liquid. It is typically sold in solid form as the sodium common salt of the acrid indicator.

Structure and properties

[edit]

Bromothymol blue
(pH indicator)
below pH 6.0 above pH 7.half dozen
vi.0 7.vi

Bromothymol blueish acts as a weak acid in a solution. Information technology tin can thus exist in protonated or deprotonated course, appearing yellow or bluish, respectively. It is bright aquamarine by itself, and dark-green-blueish in a neutral solution. The deprotonation of the neutral form results in a highly conjugated construction, accounting for the difference in color. An intermediate of the deprotonation mechanism is responsible for the greenish color in neutral solution.[two]

The protonated form of bromothymol blue has its peak absorption at 427 nm thus transmitting xanthous low-cal in acidic solutions, and the deprotonated form has its peak absorption at 602 nm thus transmitting blue lite in more than basic solutions.[3]
Highly acidic Bromothymol blue is magenta in color.

The general carbon skeleton of bromothymol blueish is common to many indicators including chlorophenol cherry-red, thymol blue, and bromocresol dark-green.[ii]

The presence of 1 moderate electron-withdrawing group (bromine cantlet) and two moderate donating groups (alkyl substituents) are responsible for bromothymol blue’s agile indication range from a pH of vi.0 to 7.6. While the conjugation is responsible for the length and nature of the colour modify range, these substituent groups are ultimately responsible for the indicator’south active range.[ii]

Construction at dissimilar pH ranges

Bromothymol bluish is sparingly soluble in oil, but soluble in water, ether, and aqueous solutions of alkalis. It is less soluble in nonpolar solvents such as benzene, toluene, and xylene, and practically insoluble in petroleum ether.[4]

Synthesis and grooming

[edit]

Bromothymol blue is synthesized by addition of elemental bromine to thymol bluish in a solution in glacial acetic acid.[5]

To prepare a solution for use as pH indicator, dissolve 0.10 thousand in 8.0 cm3
Due north/l NaOH and dilute with water to 250 cm3. To set a solution for use as indicator in volumetric work, dissolve 0.1 1000 in 100 cm3
of 50% (five/v) ethanol.[4]

Uses

[edit]

Unlike colors of bromothymol blue at marked pH conditions.

Bromothymol blue may exist used for observing photosynthetic activities, or as a respiratory indicator (turns yellow as CO2
is added).[half-dozen]
[vii]
A common demonstration of BTB’s pH indicator properties involves exhaling through a tube into a neutral solution of BTB. Equally CO2
is absorbed from the jiff into the solution, forming carbonic acrid, the solution changes color from green to yellow. Thus, BTB is commonly used in scientific discipline classes to demonstrate that the more that muscles are used, the greater the CO2
output.

Bromothymol blue has been used in conjunction with phenol cherry to monitor the fungal asparaginase enzyme activity with phenol red turning pink and bromothymol blue turning blue indicating an increase in pH and therefore enzyme activity.[8]
However, a recent study suggests that methyl cherry is more useful in determining activity due to the bright yellowish ring class in the zone of enzyme action.[9]

It may also be used in the laboratory equally a biological slide stain. At this betoken, the bromothymol is already blue, and a few drops of BTB are used on a water slide. The specimen is mixed with blue BTB solution and fixed to a slide by a cover sideslip. It is sometimes used to define cell walls or nuclei nether the microscope.

Bromothymol is used in obstetrics for detecting premature rupture of membranes.[10]
Amniotic fluid typically has a pH > 7.2, bromothymol will therefore turn blue when brought in contact with fluid leaking from the amnion. As vaginal pH normally is acidic, the bluish color indicates the presence of amniotic fluid. The examination may be false-positive in the presence of other alkaline substances such equally blood or semen, or in the presence of bacterial vaginosis.

See too

[edit]

  • pH indicator
  • Litmus
  • Phenolphthalein
  • Methyl orange
  • Universal indicator

References

[edit]


  1. ^


    “Archived copy”. Archived from the original on 2015-11-26. Retrieved
    2015-12-eleven
    .



    {{cite web}}: CS1 maint: archived re-create every bit title (link)

  2. ^


    a




    b




    c




    De Meyer, Thierry (March 2014). “Substituent effects on assimilation spectra of pH indicators: An experimental and computational written report of sulfonphthaleine dyes”.
    Dyes and Pigments.
    102: 241–250. doi:x.1016/j.dyepig.2013.ten.048. hdl:1854/LU-4353650. Archived from the original on 2020-07-29. Retrieved
    2020-03-16
    .



  3. ^


    Nahhal; et al. (18 July 2012). “Sparse film optical BTB pH sensors using sol–gel method in presence of surfactants”
    (PDF).
    International Nano Letters.
    2
    (16): 3. Bibcode:2012INL…..2…16E. doi:10.1186/2228-5326-2-16. Archived
    (PDF)
    from the original on 2014-11-29. Retrieved
    18 Nov
    2014
    .


  4. ^


    a




    b




    O’Neil, Maryadele J (2006).
    The Merck Index. Merck Enquiry Laboratory. p. 1445. ISBN978-0-911910-00-i.



  5. ^


    “Bromothymol blue”
    (PDF). Archived
    (PDF)
    from the original on 27 Nov 2020. Retrieved
    4 April
    2020
    .



  6. ^


    Sabnis R. Westward. (2007).
    Handbook of Acrid-Base of operations Indicators. CRC Press. ISBN978-0-8493-8218-5.



  7. ^


    Sabnis R. Due west. (2010).
    Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications
    (1st ed.). Wiley. ISBN978-0-470-40753-0.



  8. ^


    “Isolation and screening of 50-asparaginase free of glutaminase and urease from fungal sp”.
    researchgate.net. 22 August 2016.



    {{cite spider web}}: CS1 maint: url-status (link)


  9. ^


    Dhale, Mohan (July 2014). “A comparative rapid and sensitive method to screen l-asparaginase producing fungi”.
    Periodical of Microbiological Methods.
    102: 66–68. doi:x.1016/j.mimet.2014.04.010. PMID 24794733.



  10. ^


    King, Arthur G. (1935-12-01). “The determination of rupture of the membranes”.
    American Journal of Obstetrics & Gynecology.
    30
    (half-dozen): 860–862. doi:ten.1016/S0002-9378(35)90429-X. ISSN 0002-9378.


External links

[edit]

  • J.T.Bakery MSDS
  • Bromothymol Blue (Sodium Salt) -Sigma Aldrich



The Ph Indicator Bromothymol Blue is Blue

Source: https://en.wikipedia.org/wiki/Bromothymol_blue